Construction of a recombinant lentivirus containing human microRNA-7-3 and its inhibitory effects on...
Construction of a recombinant lentivirus containing human microRNA-7-3 and its inhibitory effects on glioma proliferation
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Author / Creator
Dong, Lun , Han, Chongxu , Zhang, Hengzhu , Gu, Xuewen , Li, Jian , Wu, Yongkang and Wang, Xiaodong
Publisher
India: Medknow Publications & Media Pvt. Ltd
Journal title
Language
English
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Publisher
India: Medknow Publications & Media Pvt. Ltd
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Contents
In the present study, we constructed a lentivirus, FIV-CMV-GFP-miR-7-3, containing the microRNA-7-3 gene and the green fluorescent protein gene, and used it to transfect human glioma U251 cells. Fluorescence microscopy showed that 80% of U251 cells expressed green fluorescence. Real-time reverse transcription PCR showed that microRNA-7-3 RNA expres...
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Full title
Construction of a recombinant lentivirus containing human microRNA-7-3 and its inhibitory effects on glioma proliferation
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Record Identifier
TN_cdi_proquest_journals_2382806825
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2382806825
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ISSN
1673-5374
E-ISSN
1876-7958
DOI
10.3969/j.issn.1673-5374.2012.27.009
