A CRISPRi-dCas9 system for archaea and its use to examine gene function during nitrogen fixation by...
A CRISPRi-dCas9 system for archaea and its use to examine gene function during nitrogen fixation by Methanosarcina acetivorans
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Cold Spring Harbor: Cold Spring Harbor Laboratory Press
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English
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Publisher
Cold Spring Harbor: Cold Spring Harbor Laboratory Press
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Contents
CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The Methanosarcina acetivorans CRISPR-Cas9 system was repurposed by replacing Cas9 with the catalytically dead Cas9 (dCas9) t...
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Full title
A CRISPRi-dCas9 system for archaea and its use to examine gene function during nitrogen fixation by Methanosarcina acetivorans
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TN_cdi_proquest_journals_2413803059
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2413803059
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E-ISSN
2692-8205
DOI
10.1101/2020.06.15.153874
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https://www.proquest.com/docview/2413803059?pq-origsite=primo&accountid=13902
