Screening of protein-ligand interactions under crude conditions by native mass spectrometry
Screening of protein-ligand interactions under crude conditions by native mass spectrometry
About this item
Full title
Author / Creator
Publisher
Berlin/Heidelberg: Springer Berlin Heidelberg
Journal title
Language
English
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Publication information
Publisher
Berlin/Heidelberg: Springer Berlin Heidelberg
Subjects
More information
Scope and Contents
Contents
A convenient analytical system for protein-ligand interactions under crude conditions was developed using native mass spectrometry (MS). As a model protein,
Escherichia coli (E. coli
) dihydrofolate reductase (DHFR) with and without a histidine tag was used for the study. First, overexpressed DHFR with a His-tag was roughly purified with a Ni...
Alternative Titles
Full title
Screening of protein-ligand interactions under crude conditions by native mass spectrometry
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Primary Identifiers
Record Identifier
TN_cdi_proquest_journals_2417702172
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2417702172
Other Identifiers
ISSN
1618-2642,1618-2650
E-ISSN
1618-2650
DOI
10.1007/s00216-020-02649-x
