Abstract Relative deficiency of TOLLIP expression in monocytes is associated with increased tuberculosis (TB) susceptibility in genetic studies, despite antagonizing host innate immune pathways that control Mycobacterium tuberculosis (Mtb) infection. In this study, we investigated the mechanisms by which TOLLIP influences Mtb immunity. Tollip-/- mice developed worsened disease, consistent with prior genetic observations, and developed large numbers of foam cells. Selective TOLLIP deletion in alveolar macrophages (AM) was sufficient to induce lipid accumulation and increased Mtb persistence 28 days after infection, despite increased antimicrobial responses. We analyzed sorted, Mtb-infected Tollip-/- AM from mixed bone marrow chimeric mice to measure global gene expression 28 days post-infection. We found transcriptional profiles consistent with increased EIF2 signaling. Selective lipid administration to Tollip-/- macrophages induced lipid accumulation, and Mtb infection of lipid laden, Tollip-/- macrophages induced cellular stress and impaired Mtb control. EIF2 activation induced increased Mtb replication within macrophages, irrespective of TOLLIP expression, and EIF2 kinases were enriched in human caseous granulomas. Our findings define a critical checkpoint for TOLLIP to prevent lipid-induced EIF2 activation and demonstrate an important mechanism for EIF2 signaling to permit Mtb replication within macrophages. Competing Interest Statement The authors have declared no competing interest. Footnotes * In this version, we added two figures to expand on the central thesis of this paper and added one author and a supplemental figure. * https://github.com/altman-lab/JS20.01 * Abbreviations AM alveolar macrophage BafA bafilomycin A CFU colony forming units DC dendritic cells DEG differentially-expressed genes EIF2 eukaryotic initiation factor 2 ER endoplasmic reticulum IM interstitial macrophages LD lipid droplet MA Mycolic acid MDM monocyte-derived macrophages Mtb Mycobacterium tuberculosis PEM peritoneal extract...

TOLLIP resolves lipid-induced EIF2 signaling in alveolar macrophages for durable Mycobacterium tuberculosis protection

10.1101/2020.08.24.263624