Diisopropylfluorophosphate (DFP) volatizes and cross-contaminates wells in a common 96-well plate ze...
Diisopropylfluorophosphate (DFP) volatizes and cross-contaminates wells in a common 96-well plate zebrafish larvae exposure method
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Cold Spring Harbor: Cold Spring Harbor Laboratory Press
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English
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Cold Spring Harbor: Cold Spring Harbor Laboratory Press
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Diisopropylfluorophosphate (DFP) is an organophosphate (OP) that is commonly used to study the neurotoxic effects of acutely intoxicating OP exposure. In preliminary studies, we discovered abnormal deaths in DMSO-only exposed larvae housed in the same plate as DFP-exposed larvae, and hypothesized that DFP volatilizes and cross-contaminates wells when using a 96-well plate exposure method for exposing zebrafish larvae. Survivability and acetylcholinesterase activity assays confirmed DFP presence in the tissues of zebrafish ostensibly exposed to DMSO only. These findings indicate DFP cross-contamination, which raises concerns for the experimental design of studies evaluating the toxicity of volatile and semi-volatile substances. Competing Interest Statement The authors have declared no competing interest. Footnotes * https://github.com/insideafish...
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Diisopropylfluorophosphate (DFP) volatizes and cross-contaminates wells in a common 96-well plate zebrafish larvae exposure method
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TN_cdi_proquest_journals_2553403160
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2553403160
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2692-8205
DOI
10.1101/2021.07.19.452994
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https://www.proquest.com/docview/2553403160?pq-origsite=primo&accountid=13902
