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Sequencing of individual barcoded cDNAs on Pacific Biosciences and Oxford Nanopore reveals platform-...

Sequencing of individual barcoded cDNAs on Pacific Biosciences and Oxford Nanopore reveals platform-...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2621424501

Sequencing of individual barcoded cDNAs on Pacific Biosciences and Oxford Nanopore reveals platform-specific error patterns

About this item

Full title

Sequencing of individual barcoded cDNAs on Pacific Biosciences and Oxford Nanopore reveals platform-specific error patterns

Publisher

Cold Spring Harbor: Cold Spring Harbor Laboratory Press

Journal title

bioRxiv, 2022-01

Language

English

Formats

Publication information

Publisher

Cold Spring Harbor: Cold Spring Harbor Laboratory Press

More information

Scope and Contents

Contents

Long-read transcriptomics requires understanding error sources inherent to technologies. Current approaches cannot compare methods for an individual RNA molecule. Here, we present a novel platform comparison method that combined barcoding strategies and long-read sequencing to sequence cDNA copies representing an individual RNA molecule on both Pac...

Alternative Titles

Full title

Sequencing of individual barcoded cDNAs on Pacific Biosciences and Oxford Nanopore reveals platform-specific error patterns

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_journals_2621424501

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2621424501

Other Identifiers

E-ISSN

2692-8205

DOI

10.1101/2022.01.17.476636