sDarken: Next generation genetically encoded fluorescent sensors for serotonin
sDarken: Next generation genetically encoded fluorescent sensors for serotonin
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Author / Creator
Kubitschke, Martin , Mueller, Monika , Wallhorn, Lutz , Pulin, Mauro , Mittag, Manuel , Pollok, Stefan , Ziebarth, Tim , Bremshey, Svenja , Gerdey, Jill , Claussen, Kristin Carolin , Renken, Kim , Gross, Juliana , Meyer, Niklas , Wiegert, Simon , Reiner, Andreas , Fuhrmann, Martin and Masseck, Olivia Andrea
Publisher
Cold Spring Harbor: Cold Spring Harbor Laboratory Press
Journal title
Language
English
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Publisher
Cold Spring Harbor: Cold Spring Harbor Laboratory Press
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Scope and Contents
Contents
We developed a new family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the...
Alternative Titles
Full title
sDarken: Next generation genetically encoded fluorescent sensors for serotonin
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Record Identifier
TN_cdi_proquest_journals_2638552210
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2638552210
Other Identifiers
E-ISSN
2692-8205
DOI
10.1101/2022.03.10.483799
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https://www.proquest.com/docview/2638552210?pq-origsite=primo&accountid=13902
