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Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structur...

Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structur...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2739272401

Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structural analyses of optogenetically-positioned organelles

About this item

Full title

Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structural analyses of optogenetically-positioned organelles

Publisher

Cold Spring Harbor: Cold Spring Harbor Laboratory Press

Journal title

bioRxiv, 2022-11

Language

English

Formats

Publication information

Publisher

Cold Spring Harbor: Cold Spring Harbor Laboratory Press

More information

Scope and Contents

Contents

Unambiguous targeting of cellular structures for in situ cryo-electron microscopy in the heterogeneous, dense, and compacted environment of the cytoplasm remains challenging. Here we have developed a novel cryogenic correlative light and electron microscopy (cryo-CLEM) workflow which combines thin cells grown on a mechanically defined substratum to...

Alternative Titles

Full title

Precision super-resolution cryo-correlative light and electron microscopy for rapid in situ structural analyses of optogenetically-positioned organelles

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_journals_2739272401

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_2739272401

Other Identifiers

E-ISSN

2692-8205

DOI

10.1101/2022.11.22.516823