Expression, One-Step Purification, and Immobilization of HaloTagTM Fusion Proteins on Chloroalkane-F...
Expression, One-Step Purification, and Immobilization of HaloTagTM Fusion Proteins on Chloroalkane-Functionalized Magnetic Beads
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Publisher
New York: Humana Press Inc
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Language
English
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Publisher
New York: Humana Press Inc
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Scope and Contents
Contents
The presented work introduces a novel method to immobilize enzymes either purified or directly out of a crude extract onto magnetic particles in the micrometer range. This method is based on the creation of a fusion protein consisting of the enzyme of choice and a mutant dehalogenase. The dehalogenase gene is commercially available from the company...
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Full title
Expression, One-Step Purification, and Immobilization of HaloTagTM Fusion Proteins on Chloroalkane-Functionalized Magnetic Beads
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TN_cdi_proquest_journals_761074756
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_761074756
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ISSN
0273-2289
E-ISSN
1559-0291
DOI
10.1007/s12010-010-8985-1
