Functional identification of hyoscyamine 6[beta]-hydroxylase from Anisodus acutangulus and overprodu...
Functional identification of hyoscyamine 6[beta]-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli
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Author / Creator
Kai, Guoyin , Liu, Yuanyuan , Wang, Xiaoyun , Yang, Sheng , Fu, Xueqing , Luo, Xiuqin and Liao, Pan
Publisher
Dordrecht: Springer Nature B.V
Journal title
Language
English
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Publisher
Dordrecht: Springer Nature B.V
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Contents
Hyoscyamine 6β-hydroxylase (H6H; EC 1.14.11.11) converts hyoscyamine to scopolamine in the last step of scopolamine biosynthetic pathway. The gene encoding H6H in Anisodus acutangulus was cloned and expressed in Escherichia coli and the recombinant proteins fused with His-tag or GST-tag at its N-terminal were purified and then confirmed by Western...
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Full title
Functional identification of hyoscyamine 6[beta]-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli
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Record Identifier
TN_cdi_proquest_journals_871775969
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_871775969
Other Identifiers
ISSN
0141-5492
E-ISSN
1573-6776
DOI
10.1007/s10529-011-0575-y
