Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
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United States: National Academy of Sciences
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Language
English
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Publisher
United States: National Academy of Sciences
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Contents
Protein interactions with peptides generally have low thermodynamic and mechanical stability. Streptococcus pyogenes fibronectin-binding protein FbaB contains a domain with a spontaneous isopeptide bond between Lys and Asp. By splitting this domain and rational engineering of the fragments, we obtained a peptide (SpyTag) which formed an amide bond...
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Full title
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin
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TN_cdi_proquest_journals_935642274
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_journals_935642274
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ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.1115485109
