Assessment of the specificity of Burkholderia and Pseudomonas qPCR assays for detection of these gen...
Assessment of the specificity of Burkholderia and Pseudomonas qPCR assays for detection of these genera in soil using 454 pyrosequencing
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Oxford, UK: Blackwell Publishing Ltd
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Language
English
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Publisher
Oxford, UK: Blackwell Publishing Ltd
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Abstract
In this study, two highly specific quantitative PCR assays targeting the bacterial genera Burkholderia and Pseudomonas were developed and evaluated on soil samples. The primers were targeting different multivariate regions of the 16S rRNA gene and designed to be compatible with quantitative PCR and the high throughput 454 pyrosequencing...
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Full title
Assessment of the specificity of Burkholderia and Pseudomonas qPCR assays for detection of these genera in soil using 454 pyrosequencing
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TN_cdi_proquest_miscellaneous_1028028939
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1028028939
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ISSN
0378-1097
E-ISSN
1574-6968
DOI
10.1111/j.1574-6968.2012.02601.x
