Overexpression in E. coli and Purification of the L. pneumophila Lpp2981 Protein
Overexpression in E. coli and Purification of the L. pneumophila Lpp2981 Protein
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Publisher
Boston: Springer-Verlag
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Language
English
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Publisher
Boston: Springer-Verlag
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Contents
The Lpp2981 gene from Legionella pneumophila, the causative agent of Legionnaire’s disease, was cloned into the pMWT7 plasmid. The construct was used to express this gene in Escherichia coli. Five different bacterial strains were tested to overexpress the gene but without success. Sequence analysis revealed a cluster of four rare codons near the 5′...
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Full title
Overexpression in E. coli and Purification of the L. pneumophila Lpp2981 Protein
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TN_cdi_proquest_miscellaneous_1500787850
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1500787850
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ISSN
1073-6085
E-ISSN
1559-0305
DOI
10.1007/s12033-013-9691-3
