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Cloning, Auto-induction Expression, and Purification of rSpaA Swine Erysipelas Antigen

Cloning, Auto-induction Expression, and Purification of rSpaA Swine Erysipelas Antigen

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1524172790

Cloning, Auto-induction Expression, and Purification of rSpaA Swine Erysipelas Antigen

About this item

Full title

Cloning, Auto-induction Expression, and Purification of rSpaA Swine Erysipelas Antigen

Publisher

New York: Springer-Verlag

Journal title

Current microbiology, 2012-10, Vol.65 (4), p.369-374

Language

English

Formats

Publication information

Publisher

New York: Springer-Verlag

More information

Scope and Contents

Contents

This work reports the cloning, expression, and purification of a 42-kDa fragment of the SpaA protein from Erysipelothrix rhusiopathiae, the main antigenic candidate for a subunit vaccine against swine erysipelas. The use of an auto-induction protocol to improve heterologous protein expression in recombinant Escherichia coli cultures was also invest...

Alternative Titles

Full title

Cloning, Auto-induction Expression, and Purification of rSpaA Swine Erysipelas Antigen

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_1524172790

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1524172790

Other Identifiers

ISSN

0343-8651

E-ISSN

1432-0991

DOI

10.1007/s00284-012-0171-y

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