Interactive assembly algorithms for molecular cloning
Interactive assembly algorithms for molecular cloning
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New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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Raven calculates assembly plans for complex genetic constructs from thousands of parts. It integrates user feedback on failed intermediate assemblies to improve the final outcome.
Molecular biologists routinely clone genetic constructs from DNA segments and formulate plans to assemble them. However, manual assembly planning is complex, error prone and not scalable. We address this problem with an algorithm-driven DNA assembly planning software tool suite called Raven (
http://www.ravencad.org/
) that produces optimized assembly plans and allows users to apply experimental outcomes to redesign assembly plans interactively. We used Raven to calculate assembly plans for thousands of variants of five types of genetic constructs, as well as hundreds of constructs of variable size and complexity from the literature. Finally, we experim...
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Interactive assembly algorithms for molecular cloning
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TN_cdi_proquest_miscellaneous_1530951874
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1530951874
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.2939
