Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multi...
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation
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England: BioMed Central Ltd
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English
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England: BioMed Central Ltd
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Arabidopsis mutants produced by constitutive overexpression of the CRISPR/Cas9 genome editing system are usually mosaics in the T1 generation. In this study, we used egg cell-specific promoters to drive the expression of Cas9 and obtained non-mosaic T1 mutants for multiple target genes with high efficiency. Comparisons of 12 combinations of eight p...
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Full title
Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation
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TN_cdi_proquest_miscellaneous_1698962359
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1698962359
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ISSN
1474-7596
E-ISSN
1474-760X
DOI
10.1186/s13059-015-0715-0
