A rapid and sensitive method for kinetic study and activity assay of DNase I in vitro based on a GO-...
A rapid and sensitive method for kinetic study and activity assay of DNase I in vitro based on a GO-quenched hairpin probe
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Author / Creator
Xu, Wei , Xie, Zhenhua , Tong, Chunyi , Peng, Lan , Xiao, Changhui , Liu, Xuanming , Zhu, Yonghua and Liu, Bin
Publisher
Berlin/Heidelberg: Springer Berlin Heidelberg
Journal title
Language
English
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Publication information
Publisher
Berlin/Heidelberg: Springer Berlin Heidelberg
Subjects
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Scope and Contents
Contents
As a waste-management endonuclease, DNase I has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis. We report here an alternative fluorescence method for DNase I assay with high accuracy and sensitivity by applying a DNA/GO (graphene oxide) probe. The method with a detection limit of 1 U mL
–1
Alternative Titles
Full title
A rapid and sensitive method for kinetic study and activity assay of DNase I in vitro based on a GO-quenched hairpin probe
Authors, Artists and Contributors
Author / Creator
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Record Identifier
TN_cdi_proquest_miscellaneous_1809626448
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1809626448
Other Identifiers
ISSN
1618-2642
E-ISSN
1618-2650
DOI
10.1007/s00216-016-9474-3
