Spliced synthetic genes as internal controls in RNA sequencing experiments
Spliced synthetic genes as internal controls in RNA sequencing experiments
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Publisher
New York: Nature Publishing Group US
Journal title
Language
English
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Publication information
Publisher
New York: Nature Publishing Group US
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Scope and Contents
Contents
Synthetic spike-in standards (‘sequins’), representing spliced mRNA isoforms, provide internal controls for assessing transcript assembly and quantification within and between RNA sequencing libraries. Sequins representing fused genes can be used to determine the sensitivity limit for oncogenic fusions in cancer samples.
RNA sequencing (RNA-seq)...
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Full title
Spliced synthetic genes as internal controls in RNA sequencing experiments
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TN_cdi_proquest_miscellaneous_1827916649
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1827916649
Other Identifiers
ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.3958
