Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs
Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs
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New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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Contents
Expressing guide RNAs from a tRNA scaffold enhances mutagenesis by both Cas9 and Cpf1 and allows conditional CRISPR applications
in vivo
.
We present tRNA-based vectors for producing multiple clustered regularly interspaced short palindromic repeats (CRISPR) single guide RNAs (sgRNAs) from a single RNA polymerase II or III transcript in
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Full title
Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs
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TN_cdi_proquest_miscellaneous_1837317538
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1837317538
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.3972
