Quantitative mRNA imaging throughout the entire Drosophila brain
Quantitative mRNA imaging throughout the entire Drosophila brain
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New York: Nature Publishing Group US
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Language
English
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Publisher
New York: Nature Publishing Group US
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Contents
Improved fluorescence
in situ
hybridization enables smFISH in cleared whole-mount
Drosophila
brains with confocal microscopy; a custom Bessel beam structured illumination microscope allows single-mRNA detection across the entire brain.
We describe a fluorescence
in situ
hybridization method that permits detection of the localiz...
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Full title
Quantitative mRNA imaging throughout the entire Drosophila brain
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TN_cdi_proquest_miscellaneous_1906139052
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1906139052
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.4309