Allosteric inhibition of individual enzyme molecules trapped in lipid vesicles
Allosteric inhibition of individual enzyme molecules trapped in lipid vesicles
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Publisher
United States: National Academy of Sciences
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Language
English
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Publisher
United States: National Academy of Sciences
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Scope and Contents
Contents
Enzymatic inhibition by product molecules is an important and widespread phenomenon. We describe an approach to study product inhibition at the single-molecule level. Individual HRP molecules are trapped within surface-tethered lipid vesicles, and their reaction with a fluorogenic substrate is probed. While the substrate readily penetrates into the...
Alternative Titles
Full title
Allosteric inhibition of individual enzyme molecules trapped in lipid vesicles
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Record Identifier
TN_cdi_proquest_miscellaneous_1999951011
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1999951011
Other Identifiers
ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.1116670109
