Large-scale, saturating insertional mutagenesis of the mouse genome
Large-scale, saturating insertional mutagenesis of the mouse genome
About this item
Full title
Author / Creator
Gragerov, Alexander , Horie, Kyoji , Pavlova, Maria , Madisen, Linda , Zeng, Hongkui , Gragerova, Galina , Rhode, Alex , Dolka, Io , Roth, Patricia , Ebbert, Amanda , Moe, Stephanie , Navas, Christopher , Finn, Eric , Bergmann, John , Vassilatis, Demetrios K , Pavlakis, George N and Gaitanaris, George A
Publisher
United States: National Academy of Sciences
Journal title
Language
English
Formats
Publication information
Publisher
United States: National Academy of Sciences
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Scope and Contents
Contents
We describe the construction of a large-scale, orderly assembly of mutant ES cells, generated with retroviral insertions and having mutational coverage in >90% of mouse genes. We also describe a method for isolating ES cell clones with mutations in specific genes of interest from this library. This approach, which combines saturating random mutagen...
Alternative Titles
Full title
Large-scale, saturating insertional mutagenesis of the mouse genome
Authors, Artists and Contributors
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Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_20010101
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_20010101
Other Identifiers
ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.0700608104
