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Endogenous Heparin Interferes with Quantification of MicroRNAs by RT-qPCR

Endogenous Heparin Interferes with Quantification of MicroRNAs by RT-qPCR

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2008884957

Endogenous Heparin Interferes with Quantification of MicroRNAs by RT-qPCR

About this item

Full title

Endogenous Heparin Interferes with Quantification of MicroRNAs by RT-qPCR

Publisher

England: Oxford University Press

Journal title

Clinical chemistry (Baltimore, Md.), 2018-05, Vol.64 (5), p.863-865

Language

English

Formats

Publication information

Publisher

England: Oxford University Press

More information

Scope and Contents

Contents

[...]heparinase treatment of RNA samples may add a supplementary bias in the quantification of miRNAs and should not be recommended. [...]our recommendation can be extrapolated to any other quantification of gene expression levels by RT-qPCR in samples potentially containing even low levels of heparin. Cardiovascular Research Unit Luxembourg Instit...

Alternative Titles

Full title

Endogenous Heparin Interferes with Quantification of MicroRNAs by RT-qPCR

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_2008884957

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2008884957

Other Identifiers

ISSN

0009-9147

E-ISSN

1530-8561

DOI

10.1373/clinchem.2017.284653

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