Construction of T-vectors for the direct, unidirectional cloning, and analysis of PCR-amplified prom...
Construction of T-vectors for the direct, unidirectional cloning, and analysis of PCR-amplified promoters
About this item
Full title
Author / Creator
Wang, B. L. , Li, X. X. , Zheng, F. , Liu, R. , Quan, J. X. , Jia, H. W. , Liang, H. , Deng, T. , Guo, S. Y. , Guo, G. , Zhang, J. Y. and Qiu, M. C.
Publisher
New York: Springer Nature B.V
Journal title
Language
English
Formats
Publication information
Publisher
New York: Springer Nature B.V
Subjects
More information
Scope and Contents
Contents
The amplification and cloning of promoters are regularly employed procedures to study the mechanism of gene regulation. In the present study we developed a method to construct T-vectors used for the direct and unidirectional cloning and analysis of promoters. These so-called T-vectors, pEGFP-T and pGL3-T, were derived from their parent promoterless...
Alternative Titles
Full title
Construction of T-vectors for the direct, unidirectional cloning, and analysis of PCR-amplified promoters
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_20680052
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_20680052
Other Identifiers
ISSN
0026-8933
E-ISSN
1608-3245
DOI
10.1134/S002689330704019X
