Determination of the Activity of 1-Deoxy-d-Xylulose 5-Phosphate Synthase by Pre-column Derivatizatio...
Determination of the Activity of 1-Deoxy-d-Xylulose 5-Phosphate Synthase by Pre-column Derivatization-HPLC Using 1,2-Diamino-4,5-Methylenedioxybenzene as a Derivatizing Reagent
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Author / Creator
Liang, Yan-Fei , Liu, Hui , Li, Heng and Gao, Wen-Yun
Publisher
New York: Springer US
Journal title
Language
English
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Publisher
New York: Springer US
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Contents
α-Ketoacids can be determined by HPLC through pre-column derivatization with 1,2-diamino-4,5-methylenedioxybenzene (DMB) as a derivatizing reagent. Using this method, the specific activity and the steady-state kinetic of 1-deoxy-
d
-xylulose-5-phosphate synthase (DXS) were measured. Firstly, DXS substrate pyruvate was derivatized with DMB in...
Alternative Titles
Full title
Determination of the Activity of 1-Deoxy-d-Xylulose 5-Phosphate Synthase by Pre-column Derivatization-HPLC Using 1,2-Diamino-4,5-Methylenedioxybenzene as a Derivatizing Reagent
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Record Identifier
TN_cdi_proquest_miscellaneous_2179486434
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2179486434
Other Identifiers
ISSN
1572-3887
E-ISSN
1573-4943,1875-8355
DOI
10.1007/s10930-019-09816-9
