An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping s...
An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast
About this item
Full title
Author / Creator
Jia, Yanrong , Ren, Ping , Duan, Shixin , Zeng, Pei , Xie, Debao and Zeng, Fanli
Publisher
Dordrecht: Springer Netherlands
Journal title
Language
English
Formats
Publication information
Publisher
Dordrecht: Springer Netherlands
Subjects
More information
Scope and Contents
Contents
Objectives
Establish a method to restrict unexpected fragments including stop codons in scFv library and generate a thermo resistant strain for screening of thermal stable scFv sequences.
Results
Here, we have constructed a T2A–Leu2 system for selection of yeast surface display libraries that blocks amplification of “stop codon” plasmids w...
Alternative Titles
Full title
An optimized yeast display strategy for efficient scFv antibody selection using ribosomal skipping system and thermo resistant yeast
Authors, Artists and Contributors
Author / Creator
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_2257700263
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2257700263
Other Identifiers
ISSN
0141-5492
E-ISSN
1573-6776
DOI
10.1007/s10529-019-02710-5
