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Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence m...

Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence m...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2429775146

Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence microscopy

About this item

Full title

Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence microscopy

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2020-09, Vol.15 (9), p.2773-2784

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Spherical aberration (SA) occurs when light rays entering at different points of a spherical lens are not focused to the same point of the optical axis. SA that occurs inside the lens elements of a fluorescence microscope is well understood and corrected for. However, SA is also induced when light passes through an interface of refractive index (RI...

Alternative Titles

Full title

Tutorial: avoiding and correcting sample-induced spherical aberration artifacts in 3D fluorescence microscopy

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_2429775146

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2429775146

Other Identifiers

ISSN

1754-2189

E-ISSN

1750-2799

DOI

10.1038/s41596-020-0360-2

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