Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density
Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density
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New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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In general, mRNAs are assumed to be loaded with ribosomes instantly upon entry into the cytoplasm. To measure ribosome density (RD) on nascent mRNA, we developed nascent Ribo-Seq by combining Ribo-Seq with progressive 4-thiouridine labeling. In mouse macrophages, we determined experimentally the lag between the appearance of nascent mRNA and its as...
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Nascent Ribo-Seq measures ribosomal loading time and reveals kinetic impact on ribosome density
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TN_cdi_proquest_miscellaneous_2569374925
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2569374925
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/s41592-021-01250-z
