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Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after...

Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2710971396

Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags

About this item

Full title

Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2022-12, Vol.17 (12), p.3056-3079

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Population behavior of signaling molecules on the cell surface is key to their adaptive function. Live imaging of proteins tagged with fluorescent molecules has been an essential tool in understanding this behavior. Typically, genetic or chemical tags are used to target molecules present throughout the cell, whereas antibody-based tags label the ex...

Alternative Titles

Full title

Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_2710971396

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2710971396

Other Identifiers

ISSN

1754-2189

E-ISSN

1750-2799

DOI

10.1038/s41596-022-00732-4

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