Quantitative detection of methylated SOCS-1, a tumor suppressor gene, by a modified protocol of quan...
Quantitative detection of methylated SOCS-1, a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection
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Dordrecht: Springer
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English
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Dordrecht: Springer
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Although methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also...
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Full title
Quantitative detection of methylated SOCS-1, a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection
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TN_cdi_proquest_miscellaneous_66963917
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_66963917
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ISSN
0141-5492
E-ISSN
1573-6776
DOI
10.1023/B:BILE.0000044922.43572.2d
