Expanded-bed adsorption immobilized-metal affinity chromatography
Expanded-bed adsorption immobilized-metal affinity chromatography
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
The protocol describes a method for capture of secreted hexahistidine-tagged proteins using expanded-bed adsorption immobilized-metal affinity chromatography. The starting material for the procedure is any crude feedstock that contains a histidine (His)-tagged target protein. The protocol is exemplified using unclarified broth from
Pichia pastor...
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Full title
Expanded-bed adsorption immobilized-metal affinity chromatography
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TN_cdi_proquest_miscellaneous_68322716
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_68322716
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ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2006.127
