Determination of absolute protein numbers in single synapses by a GFP-based calibration technique
Determination of absolute protein numbers in single synapses by a GFP-based calibration technique
About this item
Full title
Author / Creator
Publisher
New York: Nature Publishing Group US
Journal title
Language
English
Formats
Publication information
Publisher
New York: Nature Publishing Group US
Subjects
More information
Scope and Contents
Contents
To build a quantitative model of molecular organization of neurons, it is essential to have information about the number of protein molecules at individual synapses. Here we developed a method to estimate absolute numbers of individual proteins at actual excitatory synapses by calibrating the fluorescence intensity of microspheres with single EGFP...
Alternative Titles
Full title
Determination of absolute protein numbers in single synapses by a GFP-based calibration technique
Authors, Artists and Contributors
Author / Creator
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_68518743
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_68518743
Other Identifiers
ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth783
