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Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome

Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_68569866

Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome

About this item

Full title

Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome

Publisher

Washington, DC: American Association for the Advancement of Science

Journal title

Science (American Association for the Advancement of Science), 2005-09, Vol.309 (5741), p.1728-1732

Language

English

Formats

Publication information

Publisher

Washington, DC: American Association for the Advancement of Science

More information

Scope and Contents

Contents

We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. We apply this technology to resequence an evolved strain of Escherichia coli at less than one error per million consensus bases. A cell-free, mate-paired library provided s...

Alternative Titles

Full title

Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_68569866

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_68569866

Other Identifiers

ISSN

0036-8075

E-ISSN

1095-9203

DOI

10.1126/science.1117389

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