Humanization of Yeast to Produce Complex Terminally Sialylated Glycoproteins
Humanization of Yeast to Produce Complex Terminally Sialylated Glycoproteins
About this item
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Author / Creator
Hamilton, Stephen R , Davidson, Robert C , Sethuraman, Natarajan , Nett, Juergen H , Jiang, Youwei , Rios, Sandra , Bobrowicz, Piotr , Stadheim, Terrance A , Li, Huijuan , Choi, Byung-Kwon , Hopkins, Daniel , Wischnewski, Harry , Roser, Jessica , Mitchell, Teresa , Strawbridge, Rendall R , Hoopes, Jack , Wildt, Stefan and Gerngross, Tillman U
Publisher
Washington, DC: American Association for the Advancement of Science
Journal title
Language
English
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Publication information
Publisher
Washington, DC: American Association for the Advancement of Science
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More information
Scope and Contents
Contents
Yeast is a widely used recombinant protein expression system. We expanded its utility by engineering the yeast Pichia pastoris to secrete human glycoproteins with fully complex terminally sialylated N-glycans. After the knockout of four genes to eliminate yeast-specific glycosylation, we introduced 14 heterologous genes, allowing us to replicate th...
Alternative Titles
Full title
Humanization of Yeast to Produce Complex Terminally Sialylated Glycoproteins
Authors, Artists and Contributors
Author / Creator
Davidson, Robert C
Sethuraman, Natarajan
Nett, Juergen H
Jiang, Youwei
Rios, Sandra
Bobrowicz, Piotr
Stadheim, Terrance A
Li, Huijuan
Choi, Byung-Kwon
Hopkins, Daniel
Wischnewski, Harry
Roser, Jessica
Mitchell, Teresa
Strawbridge, Rendall R
Hoopes, Jack
Wildt, Stefan
Gerngross, Tillman U
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_68843767
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_68843767
Other Identifiers
ISSN
0036-8075
E-ISSN
1095-9203
DOI
10.1126/science.1130256
