A heat shock following electroporation induces highly efficient transformation of Corynebacterium gl...
A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA
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Publisher
Berlin: Springer
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Language
English
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Berlin: Springer
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Contents
An improved method for the electrotransformation of wild-type Corynebacterium glutamicum (ATCC 13032) is described. The two crucial alterations to previously developed methods are: cultivation of cells used for electrotransformation at 18 degrees C instead of 30 degrees C, and application of a heat shock immediately following electrotransformation....
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A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA
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TN_cdi_proquest_miscellaneous_69293607
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_69293607
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ISSN
0175-7598
E-ISSN
1432-0614
DOI
10.1007/s002530051557
