PCR-amplification of GC-rich regions: 'slowdown PCR'
PCR-amplification of GC-rich regions: 'slowdown PCR'
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
The polymerase chain reaction (PCR) technique has become an indispensable method in molecular research. However, PCR-amplification of GC-rich templates is often hampered by the formation of secondary structures like hairpins and higher melting temperatures. We present a novel method termed 'Slowdown PCR', which allows the successful PCR-amplificati...
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Full title
PCR-amplification of GC-rich regions: 'slowdown PCR'
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TN_cdi_proquest_miscellaneous_69451572
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_69451572
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ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2008.112
