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Studying Interactions of Four Proteins in the Yeast Two-Hybrid System: Structural Resemblance of the...

Studying Interactions of Four Proteins in the Yeast Two-Hybrid System: Structural Resemblance of the...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_69973791

Studying Interactions of Four Proteins in the Yeast Two-Hybrid System: Structural Resemblance of the pVHL/Elongin BC/hCUL-2 Complex with the Ubiquitin Ligase Complex SKP1/Cullin/F-Box Protein

About this item

Full title

Studying Interactions of Four Proteins in the Yeast Two-Hybrid System: Structural Resemblance of the pVHL/Elongin BC/hCUL-2 Complex with the Ubiquitin Ligase Complex SKP1/Cullin/F-Box Protein

Publisher

United States: National Academy of Sciences of the United States of America

Journal title

Proceedings of the National Academy of Sciences - PNAS, 1999-08, Vol.96 (17), p.9533-9538

Language

English

Formats

Publication information

Publisher

United States: National Academy of Sciences of the United States of America

More information

Scope and Contents

Contents

The yeast two-hybrid system is a powerful technique that detects interactions between two proteins and has been useful in identifying new binding partners. However, the system fails to detect protein-protein interactions that require the presence of additional components of a multisubunit complex. Here we demonstrate that the vector YIpDCE1 can be...

Alternative Titles

Full title

Studying Interactions of Four Proteins in the Yeast Two-Hybrid System: Structural Resemblance of the pVHL/Elongin BC/hCUL-2 Complex with the Ubiquitin Ligase Complex SKP1/Cullin/F-Box Protein

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_69973791

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_69973791

Other Identifiers

ISSN

0027-8424

E-ISSN

1091-6490

DOI

10.1073/pnas.96.17.9533

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