Studying Interactions of Four Proteins in the Yeast Two-Hybrid System: Structural Resemblance of the...
Studying Interactions of Four Proteins in the Yeast Two-Hybrid System: Structural Resemblance of the pVHL/Elongin BC/hCUL-2 Complex with the Ubiquitin Ligase Complex SKP1/Cullin/F-Box Protein
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United States: National Academy of Sciences of the United States of America
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Language
English
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United States: National Academy of Sciences of the United States of America
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The yeast two-hybrid system is a powerful technique that detects interactions between two proteins and has been useful in identifying new binding partners. However, the system fails to detect protein-protein interactions that require the presence of additional components of a multisubunit complex. Here we demonstrate that the vector YIpDCE1 can be...
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Full title
Studying Interactions of Four Proteins in the Yeast Two-Hybrid System: Structural Resemblance of the pVHL/Elongin BC/hCUL-2 Complex with the Ubiquitin Ligase Complex SKP1/Cullin/F-Box Protein
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TN_cdi_proquest_miscellaneous_69973791
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_69973791
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ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.96.17.9533