Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
About this item
Full title
Author / Creator
Publisher
New York: Nature Publishing Group US
Journal title
Language
English
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Publisher
New York: Nature Publishing Group US
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Scope and Contents
Contents
We describe a new cloning method, sequence and ligation–independent cloning (SLIC), which allows the assembly of multiple DNA fragments in a single reaction using
in vitro
homologous recombination and single-strand annealing. SLIC mimics
in vivo
homologous recombination by relying on exonuclease-generated ssDNA overhangs in insert and v...
Alternative Titles
Full title
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
Authors, Artists and Contributors
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TN_cdi_proquest_miscellaneous_70214035
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_70214035
Other Identifiers
ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth1010
