A genomic integration method to visualize localization of endogenous mRNAs in living yeast
A genomic integration method to visualize localization of endogenous mRNAs in living yeast
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New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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Contents
mRNA localization may be an important determinant for protein localization. We describe a simple PCR-based genomic-tagging strategy (m-TAG) that uses homologous recombination to insert binding sites for the RNA-binding MS2 coat protein (MS2-CP) between the coding region and 3′ untranslated region (UTR) of any yeast gene. Upon coexpression of MS2-CP...
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Full title
A genomic integration method to visualize localization of endogenous mRNAs in living yeast
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TN_cdi_proquest_miscellaneous_70441235
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_70441235
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth1040
