Characterization of a dITPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and it...
Characterization of a dITPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in PCR amplification
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Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag
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English
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Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag
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In this study, we found that deoxyinosine triphosphate (dITP) could inhibit polymerase chain reaction (PCR) amplification of various family B-type DNA polymerases, and 0.93% dITP was spontaneously generated from deoxyadenosine triphosphate during PCR amplification. Thus, it was hypothesized that the generated dITP might have negative effect on PCR...
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Full title
Characterization of a dITPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in PCR amplification
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TN_cdi_proquest_miscellaneous_70775828
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_70775828
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ISSN
0175-7598
E-ISSN
1432-0614
DOI
10.1007/s00253-008-1467-5
