A simple and rapid Agrobacterium -mediated transformation protocol for cotton ( Gossypium hirsutum L...
A simple and rapid Agrobacterium -mediated transformation protocol for cotton ( Gossypium hirsutum L.): Embryogenic calli as a source to generate large numbers of transgenic plants
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Publisher
Germany: Springer Nature B.V
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Language
English
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Publisher
Germany: Springer Nature B.V
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Contents
A protocol is presented for efficient transformation and regeneration of cotton. Embryogenic calli co-cultivated with Agrobacterium carrying cry1Ia5 gene were cultured under dehydration stress and antibiotic selection for 3-6 weeks to generate several transgenic embryos. An average of 75 globular embryo clusters were observed on selection plates an...
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Full title
A simple and rapid Agrobacterium -mediated transformation protocol for cotton ( Gossypium hirsutum L.): Embryogenic calli as a source to generate large numbers of transgenic plants
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TN_cdi_proquest_miscellaneous_71737487
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_71737487
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ISSN
0721-7714
E-ISSN
1432-203X
DOI
10.1007/s00299-003-0710-x
