Simple Technique for Internal Control of Real-Time Amplification Assays
Simple Technique for Internal Control of Real-Time Amplification Assays
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Washington, DC: Am Assoc Clin Chem
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English
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Washington, DC: Am Assoc Clin Chem
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Background: In real-time PCR assays, the most accurate way to identify false-negative results, e.g., those caused by PCR inhibitors, is to add to samples an internal control that will be coamplified with the target (e.g., pathogen) DNA. Current internal control procedures, however, which usually involve the introduction of a DNA fragment, are compl...
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Simple Technique for Internal Control of Real-Time Amplification Assays
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TN_cdi_proquest_miscellaneous_71853442
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_71853442
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ISSN
0009-9147
E-ISSN
1530-8561
DOI
10.1373/clinchem.2003.027961