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Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents f...

Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents f...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_734184522

Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents from Bacillus megaterium

About this item

Full title

Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents from Bacillus megaterium

Publisher

Basel, Switzerland: S. Karger AG

Journal title

Journal of molecular microbiology and biotechnology, 2009-01, Vol.17 (4), p.188-200

Language

English

Formats

Publication information

Publisher

Basel, Switzerland: S. Karger AG

More information

Scope and Contents

Contents

A tyrosinase-expressing bacterium was isolated from soil, and extracellular enzymatic activity was induced by the presence of tyrosine and CuSO 4 . Amplification of the 16S rDNA genes revealed a high similarity with Bacillus megaterium. The enzyme was over-expressed in Escherichia coli BL21 and purified using an affinity column. The tyrosinase was...

Alternative Titles

Full title

Isolation, Cloning and Characterization of a Tyrosinase with Improved Activity in Organic Solvents from Bacillus megaterium

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_734184522

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_734184522

Other Identifiers

ISSN

2673-1665,1464-1801

E-ISSN

2673-1673,1660-2412

DOI

10.1159/000233506

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