Cloning of Circular DNAs from Microorganisms Using a Novel Plasmid Capture System
Cloning of Circular DNAs from Microorganisms Using a Novel Plasmid Capture System
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Publisher
New York: New York : Humana Press Inc
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Language
English
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Publisher
New York: New York : Humana Press Inc
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Scope and Contents
Contents
Plasmid capture system (PCS) facilitates cloning and manipulation of circular double-stranded DNA. We recently developed an improved PCS (PCS-LZ) to clone relatively large DNA molecules of 30-150 kb. The PCS-LZ donor consists of a mini-F replicon and a kanamycin resistance marker between Tn7 left and Tn7 right ends. Both the replicon and marker gen...
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Full title
Cloning of Circular DNAs from Microorganisms Using a Novel Plasmid Capture System
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TN_cdi_proquest_miscellaneous_745607499
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_745607499
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ISSN
1073-6085
E-ISSN
1559-0305
DOI
10.1007/s12033-009-9215-3
