Expression, One-Step Purification, and Immobilization of HaloTag™ Fusion Proteins on Chloroalkane-Fu...
Expression, One-Step Purification, and Immobilization of HaloTag™ Fusion Proteins on Chloroalkane-Functionalized Magnetic Beads
About this item
Full title
Author / Creator
Publisher
Heidelberg: Springer
Journal title
Language
English
Formats
Publication information
Publisher
Heidelberg: Springer
Subjects
More information
Scope and Contents
Contents
The presented work introduces a novel method to immobilize enzymes either purified or directly out of a crude extract onto magnetic particles in the micrometer range. This method is based on the creation of a fusion protein consisting of the enzyme of choice and a mutant dehalogenase. The dehalogenase gene is commercially available from the company...
Alternative Titles
Full title
Expression, One-Step Purification, and Immobilization of HaloTag™ Fusion Proteins on Chloroalkane-Functionalized Magnetic Beads
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_755971501
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_755971501
Other Identifiers
ISSN
0273-2289
E-ISSN
1559-0291
DOI
10.1007/s12010-010-8985-1
