Lipase‐based quantitation of triacylglycerols in cellular lipid extracts: Requirement for presence o...
Lipase‐based quantitation of triacylglycerols in cellular lipid extracts: Requirement for presence of detergent and prior separation by thin‐layer chromatography
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Author / Creator
Publisher
Berlin/Heidelberg: Springer‐Verlag
Journal title
Language
English
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Publisher
Berlin/Heidelberg: Springer‐Verlag
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Scope and Contents
Contents
A protocol, based on the use of Pseudomonas lipase, is presented to measure quantitatively the amount of triacylglycerols in extracts from cultured cells or tissues. Since the lipase also acts on di‐ and monoacylglycerols, separation of the extracts by thin‐layer chromatography is recommended. In order to allow the lipase‐catalyzed hydrolysis to pr...
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Full title
Lipase‐based quantitation of triacylglycerols in cellular lipid extracts: Requirement for presence of detergent and prior separation by thin‐layer chromatography
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Author / Creator
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Record Identifier
TN_cdi_proquest_miscellaneous_79523369
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_79523369
Other Identifiers
ISSN
0024-4201
E-ISSN
1558-9307
DOI
10.1007/s11745-006-0166-1
