Creation of genome-wide protein expression libraries using random activation of gene expression
Creation of genome-wide protein expression libraries using random activation of gene expression
About this item
Full title
Author / Creator
Harrington, John J. , Sherf, Bruce , Rundlett, Stephen , Jackson, P. David , Perry, Rob , Cain, Scott , Leventhal, Christina , Thornton, Mark , Ramachandran, Rakesh , Whittington, Jessica , Lerner, Laura , Costanzo, Dana , McElligott, Karen , Boozer, Sherry , Mays, Robert , Smith, Emery , Veloso, Neil , Klika, Alison , Hess, Jennifer , Cothren, Kevin , Lo, Kalok , Offenbacher, Jason , Danzig, Joel and Ducar, Matt
Publisher
New York: Nature Publishing Group US
Journal title
Language
English
Formats
Publication information
Publisher
New York: Nature Publishing Group US
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More information
Scope and Contents
Contents
Here we report the use of random activation of gene expression (RAGE) to create genome-wide protein expression libraries. RAGE libraries containing only 5 × 10
6
individual clones were found to express every gene tested, including genes that are normally silent in the parent cell line. Furthermore, endogenous genes were activated at similar f...
Alternative Titles
Full title
Creation of genome-wide protein expression libraries using random activation of gene expression
Authors, Artists and Contributors
Author / Creator
Sherf, Bruce
Rundlett, Stephen
Jackson, P. David
Perry, Rob
Cain, Scott
Leventhal, Christina
Thornton, Mark
Ramachandran, Rakesh
Whittington, Jessica
Lerner, Laura
Costanzo, Dana
McElligott, Karen
Boozer, Sherry
Mays, Robert
Smith, Emery
Veloso, Neil
Klika, Alison
Hess, Jennifer
Cothren, Kevin
Lo, Kalok
Offenbacher, Jason
Danzig, Joel
Ducar, Matt
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_858422501
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_858422501
Other Identifiers
ISSN
1087-0156
E-ISSN
1546-1696
DOI
10.1038/88107
