Polymerase reaction without primers throughout for the reconstruction of full-length cDNA from produ...
Polymerase reaction without primers throughout for the reconstruction of full-length cDNA from products of rapid amplification of cDNA ends (RACE)
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Publisher
Dordrecht: Springer-Verlag
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Language
English
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Publisher
Dordrecht: Springer-Verlag
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Contents
Rapid amplification of cDNA ends (RACE) has widely been used to determine both ends of the cDNA from its partial sequence. Conventionally, 5′- and 3′-RACE products were ligated at a restriction site in the overlap region to reconstruct the full-length cDNA; however, reconstruction is difficult if no appropriate restriction enzymes are available. He...
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Full title
Polymerase reaction without primers throughout for the reconstruction of full-length cDNA from products of rapid amplification of cDNA ends (RACE)
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TN_cdi_proquest_miscellaneous_876242724
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_876242724
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ISSN
0141-5492
E-ISSN
1573-6776
DOI
10.1007/s10529-011-0580-1
