Functional identification of hyoscyamine 6β-hydroxylase from Anisodus acutangulus and overproduction...
Functional identification of hyoscyamine 6β-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli
About this item
Full title
Author / Creator
Kai, Guoyin , Liu, Yuanyuan , Wang, Xiaoyun , Yang, Sheng , Fu, Xueqing , Luo, Xiuqin and Liao, Pan
Publisher
Dordrecht: Springer Netherlands
Journal title
Language
English
Formats
Publication information
Publisher
Dordrecht: Springer Netherlands
Subjects
More information
Scope and Contents
Contents
Hyoscyamine 6β-hydroxylase (H6H; EC 1.14.11.11) converts hyoscyamine to scopolamine in the last step of scopolamine biosynthetic pathway. The gene encoding H6H in
Anisodus acutangulus
was cloned and expressed in
Escherichia coli
and the recombinant proteins fused with His-tag or GST-tag at its
N
-terminal were purified and then co...
Alternative Titles
Full title
Functional identification of hyoscyamine 6β-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli
Authors, Artists and Contributors
Author / Creator
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_876249517
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_876249517
Other Identifiers
ISSN
0141-5492
E-ISSN
1573-6776
DOI
10.1007/s10529-011-0575-y
