Large-scale plasmid preparation for transient gene expression
Large-scale plasmid preparation for transient gene expression
About this item
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Publisher
Dordrecht: Springer-Verlag
Journal title
Language
English
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Publication information
Publisher
Dordrecht: Springer-Verlag
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Scope and Contents
Contents
Large-scale transient gene expression of recombinant protein in mammalian cells requires a great amount of plasmid. An economical method for large-scale plasmid preparation, based on fed-batch fermentation and an improved plasmid extraction process, has been established. Fed-batch growth of E. coli was carried out in 5 l bioreactor by controlling t...
Alternative Titles
Full title
Large-scale plasmid preparation for transient gene expression
Authors, Artists and Contributors
Author / Creator
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Record Identifier
TN_cdi_proquest_miscellaneous_883017012
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_883017012
Other Identifiers
ISSN
0141-5492,1573-6776
E-ISSN
1573-6776
DOI
10.1007/s10529-011-0612-x
